The Rotarod is the golden standard for assessing motor performance, coordination, and learning in mice. A rodent (mouse or rat) is placed on a rotating cylinder, and the rotation speed gradually increases. Motor performance is measured by the maximal RPM (rounds per minute) at which the rodent can keep up with the rotating rod as well as latency (how long can the rodent stay on the rod before falling off). Motor learning can be assessed by training mice on the rod for several trials.

In preclinical neurology research, quantifying motor performance is crucial, particularly when assessing candidate compounds’ efficacy on conditions like Parkinson’s disease, amyotrophic lateral sclerosis (ALS), multiple sclerosis, or even studying drug-induced side effects. This test allows you to observe how well a rodent can maintain balance on a rotating cylinder, revealing motor deficits or improvements over time. As part of InnoSer’s innovative preclinical neurology CRO solutions, rotarod is commonly used in combination with several other motor tests, such as Grip Strength test, Balance Beam, or CatWalk automated gait analysis system. However, as each motor function test is unique, targeting different motor function skills in rodents, we recommend you discuss the most appropriate motor function behavioral test battery with our neurology study directors.