Metabolic disease models – MASHÂ Mouse Model
Advance your MAFLD/MASH research with the diet-induced MASH NASH mouse model Â
MASH Mouse Model Characteristics
The MASH / NASH mouse model is a diet-induced mouse model leading to development of key hallmarks of (non-alcoholic fatty liver disease) NAFLD. Male mice (C57BL/6NTac) are conditioned on a modified Amylin liver NASH (AMLN) diet, containing 40% fat, 20% fructose and 2% cholesterol. This leads to the development of progressive obesity, fatty liver, liver inflammation, fibrosis, steatosis, hepatocyte ballooning, and activated stellate cells. Â
The diet-induced phenotype of NASH mice combined with InnoSer’s imaging, biochemical and histopathology capabilities offer you a robust platform for testing efficacy of novel, potential therapies targeting liver health, metabolic dysfunction, and inflammation. This model is ideal for studying NASH progression and evaluating potential therapeutic interventions, providing a range of histopathology and biochemical markers relevant to human NASH. Contact us today to discuss how this model can advance your research in NASH and NAFLD. Â
✓ A modified AMLN diet leads to progressive development of MAFLD/MASH (NASH/NAFLD) in C57BL/6NTac.  Â
✓ Disease progression validated at InnoSer at different ages revealing progressive worsening of disease phenotype. Â

As a preclinical metabolic CRO, InnoSer’s team delivers you with a range of high-quality in vivo models for key metabolic diseases with different associated risk factors and aetiologias. This selection allows you to test therapeutic efficacy to prevent or reverse insulin resistance, pre-diabetes, obesity type 2 diabetes mellitus (T2DM), and/or MAFLD/MASH (NASH/NAFLD). To study metabolic disease-associated cardiovascular complications and cardiac dysfunction, InnoSer recommends the use of the Western diet-induced diabetic cardiomyopathy rat model. Reach out to our team to discuss the most suitable model to answer your research questions. Â
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MASH Mouse Model Sample Data

The diet-induced MASH NASH mouse model is suitable to obtain initial efficacy data in a physiologically induced model of MAFLD/MASH NASH/NAFLD.
Histopathology analyses reveal macrovascular steatosis (dotted line arrow), microvascular steatosis (bold arrow) and inflammatory cell clusters (within circles) at different ages of disease progression.

The diet-induced MASH NASH mouse model is suitable to obtain initial efficacy data in a physiologically induced model of MAFLD/MASH NASH/NAFLD.
Histopathological analyses reveal fibrosis at different ages of disease progression.Â

The diet-induced NASH mouse model is suitable to obtain initial efficacy data in a physiologically induced model of NASH/NAFLD.
The hepatic/Renal ratio, assessed by ultrasound, is increased in DIO C57BL6J MASH NASH animals indicating liver steatosis. Â

The diet-induced NASH mouse model is suitable to obtain initial efficacy data in a physiologically induced model of NASH/NAFLD.
Creatinine kinase is significantly higher in C57BL6J-NASH animals compared to healthy controls at different ages of disease progression.

The diet-induced NASH mouse model is suitable to obtain initial efficacy data in a physiologically induced model of NASH/NAFLD.
Liver function parameters (ALT, AST) are significantly higher in C57BL6J-MASH NASH animals compared to healthy controls at different ages of disease progression.Â

The diet-induced NASH mouse model is suitable to obtain initial efficacy data in a physiologically induced model of NASH/NAFLD.
Cholesterol levels are significantly higher in C57BL6J-NASH animals compared to healthy controls at different ages of disease progression.Â

The diet-induced NASH mouse model is suitable to obtain initial efficacy data in a physiologically induced model of NASH/NAFLD.
Blood glucose levels are higher in C57BL6J NASH mice compared to healthy controls (IPGTT).
MASH Mouse Model Readouts
The People Behind Your Research
Yanick Fanton, PhD, Chief Scientific Officer
As Chief Science Officer at InnoSer, Yanick is responsible for all customer studies at InnoSer and takes care of the scientific and technical coordination.
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