Metabolic disease models – MASH Mouse Model

Advance your MAFLD/MASH research with the diet-induced MASH NASH mouse model  

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MASH Mouse Model Characteristics

The MASH / NASH mouse model is a diet-induced mouse model leading to development of key hallmarks of (non-alcoholic fatty liver disease) NAFLD. Male mice (C57BL/6NTac) are conditioned on a modified Amylin liver NASH (AMLN) diet, containing 40% fat, 20% fructose and 2% cholesterol. This leads to the development of progressive obesity, fatty liver, liver inflammation, fibrosis, steatosis, hepatocyte ballooning, and activated stellate cells.  

The diet-induced phenotype of NASH mice combined with InnoSer’s imaging, biochemical and histopathology capabilities offer you a robust platform for testing efficacy of novel, potential therapies targeting liver health, metabolic dysfunction, and inflammation. This model is ideal for studying NASH progression and evaluating potential therapeutic interventions, providing a range of histopathology and biochemical markers relevant to human NASH. Contact us today to discuss how this model can advance your research in NASH and NAFLD.  

✓  A modified AMLN diet leads to progressive development of MAFLD/MASH (NASH/NAFLD) in C57BL/6NTac.   

✓  Disease progression validated at InnoSer at different ages revealing progressive worsening of disease phenotype.  

MASH mouse model

As a preclinical metabolic CRO, InnoSer’s team delivers you with a range of high-quality in vivo models for key metabolic diseases with different associated risk factors and aetiologias. This selection allows you to test therapeutic efficacy to prevent or reverse insulin resistance, pre-diabetes, obesity type 2 diabetes mellitus (T2DM), and/or MAFLD/MASH (NASH/NAFLD). To study metabolic disease-associated cardiovascular complications and cardiac dysfunction, InnoSer recommends the use of the Western diet-induced diabetic cardiomyopathy rat model. Reach out to our team to discuss the most suitable model to answer your research questions.  

Uncover Diet-Induced Cognitive Deficits.

Explore key findings on how HF and HFCF diets impact cognition, with detailed data on memory performance and metabolic effects in mice. Download the poster for full insights.

MASH cognitive deficits poster

MASH Mouse Model Sample Data

MASH Mouse Model Readouts

Key MASH mouse model readouts that InnoSer offers


Test the efficacy of your treatments in the following battery of translational readouts:
  • Body weight assessment
  • Ultrasound imaging (hepatic renal ratio, portal vein size, renal flow, echocardiography)
  • Blood biochemistry (blood cholesterol, triglyceride, ALT/AST/GGT, creatine kinase, troponin, insulin, glucagon)
  • Glucose tolerance test (IPGTT)
  • Organ collection, weighing, and processing for histopathology analyses
  • Histopathology (H&E, Pico Sirius Red, Masson’s Trichrome, alpha-SMA, Collagen, liver immune cell cytokine analysis)   

The People Behind Your Research

Yanick Fanton, PhD, Chief Scientific Officer

As Chief Science Officer at InnoSer, Yanick is responsible for all customer studies at InnoSer and takes care of the scientific and technical coordination.

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AAALAC Accreditation

InnoSer has earned the AAALAC accreditation, demonstrating our commitment to responsible animal care and use. AAALAC International is a nonprofit organization that promotes the humane treatment of animals in science through voluntary accreditation and assessment programs. Our accreditation is valid for three years, incl. 2023. Read more about the AAALAC accreditation programme here.

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Animal Welfare

The 3Rs impact everything from policy and regulatory change to the development and uptake of new technologies and approaches. This is why InnoSer has ongoing commitment and monitoring of these processes. The steps we practice maximize our ability to replace, reduce and refine animal involvement and facilitate our commitment to these principles when it comes to research and drug development.

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