Multiple Sclerosis – Experimental Autoimmune Encephalomyelitis EAE Mouse Model

Investigate your compound’s efficacy on the inflammation and immune component of Multiple Sclerosis using the EAE mouse model 

Home » Immunology CRO services » Experimental Autoimmune Encephalomyelitis (EAE) Mouse Model

 EAE Mouse Model Key Characteristics

Experimental autoimmune encephalomyelitis (EAE) mouse model is a relevant preclinical model to study efficacy of novel treatments against multiple sclerosis. The EAE mouse model replicates the key pathological and clinical features of human multiple sclerosis (MS), characterized by autoimmune-induced neuroinflammation, astrogliosis and axonal damage that manifest as motor, sensory, autonomic and cognitive disabilities.  

EAE mouse model is established either by immunization (referred to as active EAE mouse model) or by T-cell transfer from mice immunized for active EAE (referred to as adoptive transfer EAE mouse model). Active EAE mouse model is induced by immunization with antigens such as myelin-oligodendrocyte protein (MOG), myelin basic protein (MBP) or proteolipid protein (PLP) together with Complete Freund’s adjuvant (CFA) accompanied by an intraperitoneal injection of pertussis toxin (PTX) on the day of immunization and two days later. Immunization via active EAE induction can also be set-up in rats. For the adoptive transfer model, donor mice are first immunized with CNS antigen, followed by splenocyte isolation with in vitro re-activation, and the transfer of encephalitogenic T-cells to acceptor mice.  

✓  Peripheral inflammatory infiltrate (lymphocytes, macrophage) induced CNS inflammation, nerve injury, axon cell degeneration, and cell death. 

✓  Progressive limb paralysis. 

✓  Complementary PK/PD profiling services. 

EAE mouse model

As a preclinical immunology CRO, InnoSer offers well-established and clinically relevant multiple sclerosis mouse models, complemented with standardized study protocols to ensure consistency and reproducibility of your results. In addition, InnoSer offers research services using the Cuprizone, and Lysolecithin mouse models. While the EAE mouse model allows you to focus on the inflammatory component of MS, the Cuprizone and Lysolecithin models enable you to test the efficacy of compounds directed at remyelination. However, as all models have their unique characteristics, we recommend discussing your study setup in close collaboration with our experts.  

InnoSer’s research network comprises scientific experts working on multiple sclerosis, offering you the possibility to consult your compound’s MOA in-depth.

EAE Mouse Model Sample Data

EAE Mouse Model Readouts

Key EAE mouse model readouts that InnoSer offers


Test the efficacy of your treatments in the following battery of translational readouts:

  • Body weight assessment 
  • Daily EAE neurological disease scoring  
  • Spatial memory assessment (e.g., Y maze) 
  • Skeletal muscle strength assessment (e.g., grip strength, wire hang test) 
  • Motor function assessment (Rotarod, compound muscle action potential [CMAP])  
  • Biomarker analysis in plasma (e.g., neurofilament light chain [NfL], inflammatory cytokines) and CSF (e.g., auto-antigens for MOG, MBP, PLP, inflammatory cytokines) 
  • Myelin layer thickness (TEM G-ratio)  
  • Brain histopathology to assess the degree of myelin, peripheral inflammation, microgliosis, astrocyte activation, and/or axonal degeneration  
  • Blood collection for PK/PD profiling  

The People Behind Your Research

Jolien Beekens, PhD

Hasselt University/BIOMED

As part of a joint initiative to advance preclinical MS research, InnoSer works together with researchers from BIOMED, who focus on immunological mechanisms, myelination, and damage processes in the brain during MS. 

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InnoSer has earned the AAALAC accreditation, demonstrating our commitment to responsible animal care and use. AAALAC International is a nonprofit organization that promotes the humane treatment of animals in science through voluntary accreditation and assessment programs. Our accreditation is valid for three years, incl. 2023. Read more about the AAALAC accreditation programme here.

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Animal Welfare

The 3Rs impact everything from policy and regulatory change to the development and uptake of new technologies and approaches. This is why InnoSer has ongoing commitment and monitoring of these processes. The steps we practice maximize our ability to replace, reduce and refine animal involvement and facilitate our commitment to these principles when it comes to research and drug development.