Seed and Spread Tauopathy Model
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Key characteristics
- Unilateral stereotactic injection of hyperphosphorylated brainstem lysate or synthetic K18 preformed fibrils into the hippocampus
- Progressive seeding and spreading of Tau pathology
- Increased microgliosis and astrocytosis
- Correlation between ipsi- and contralateral hippocampus
Successful seed and spreading of Tau pathology after unilateral injection of brain lysate of either old TauP301S mice with high levels of phosphorylated and aggregated Tau (Tau aggregates, n = 9) or from age-matched wild types (negative control, n =5) into the hippocampus. Stereotactic injection was performed at an age of 2 months. Pathological Tau particles (AT8) were quantified 9 weeks after injection, in both the ipsilateral (left) and contralateral (right) hippocampus as measured by IHC (mean ± SEM). Statistics: Welches’ unpaired t test.
Time course of Tau seeding and spreading after unilateral injection of brain lysate of either old TauP301L mice with high levels of phosphorylated and aggregated Tau (Tau aggregates, n = 8) or from age-matched wild types (negative control, n =6) into the hippocampus. Stereotactic injection was performed at an age of 3.2 months. Pathological Tau particles (AT8) were quantified 2, 3, 5, 9 and 13 weeks (control only at 13 weeks) after injection, in both the ipsilateral (left) and contralateral (right) hippocampus as measured by IHC (mean ± SEM). Statistics: Welches’ unpaired t test.
Literature
Describing papers:
- Original paper: Cfr TAU[P301S] or TAU[P301L]
- Detrez et al., 2019: Regional vulnerability and spreading of hyperphosphorylated tau in seeded mouse brain. DOI: 10.1016/j.nbd.2019.03.010
Services
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Readouts & Analyses
- Full suite of histopathology services
- Immunohistochemistry of brain and nerve tissues (IHC)
- Bioanalyses in various biological matrices
- Flow Cytometry
- ELISA, Ultra-Sensitive ELISA for CSF/plasma biomarkers
- Meso Scale Discovery (MSD)
- Immunoblotting
In Vivo Analyses
- Mouse breeding and genotyping
- Microbiome transplantation studies
- Compound administration
- Stereotaxic surgery
- EEG, EMG and ECG analyses
- Cannula implantation
- Tissue collection and analysis
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