Seed and spread alpha-synuclein model
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Key characteristics
- Unilateral stereotactic injection of sonicated mPFFs into the dorsal striatum
- Succesful seeding and progressive spreading of alpha-synuclein pathology to other brain regions, including substantia nigra
- Dopaminergic cell loss and microgliosis
Time course of alpha-synuclein seeding and resulting loss of dopaminergic terminals after unilateral injection of sonicated murine pre-formed fibrils or PBS into the dorsal striatum. Stereotactic injection was performed at an age of 1.8 months. Pathological alpha-synuclein (pSer129-alpha-Syn) and loss of dopaminergic terminals (TH) were quantified 5, 9 and 13 weeks (control only at 9 weeks) after injection in the striatum as measured by IHC (mean ± SEM, n = 8). Statistics: Tukey’s One-Way ANOVA. Scale bar is 200 µm.
Amytracker 520 (Ebba Biotech) staining to pick up amyloidogenic structures 9 weeks post-injection in the dorsal striatum (left). The alpha-synuclein pathology spreads further to the amygdala (right). Amytracker 520 detect both Lewy Bodies and Lewy Neurites. Scale bar is 100 µm.
Time course of alpha-synuclein spreading to the contralateral striatum after injection of sonicated murine pre-formed fibrils or PBS into the ipsilateral dorsal striatum. Stereotactic injection was performed at an age of 1.8 months. Pathological alpha-synuclein (pSer129-alpha-Syn) was quantified 5, 9, and 13 weeks (control only at 9 weeks) after injection in the striatum as measured by IHC (mean ± SEM, n = 8). Statistics: Tukey’s One-Way ANOVA.
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Readouts & Analyses
- Full suite of histopathology services
- Immunohistochemistry of brain and nerve tissues (IHC)
- Bioanalyses in various biological matrices
- Flow Cytometry
- ELISA, Ultra-Sensitive ELISA for CSF/plasma biomarkers
- Meso Scale Discovery (MSD)
- Immunoblotting
In Vivo Analyses
- Mouse breeding and genotyping
- Microbiome transplantation studies
- Compound administration
- Stereotaxic surgery
- EEG, EMG and ECG analyses
- Cannula implantation
- Tissue collection and analysis
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